CoreBio RNA Prep - Library Preparation Kit (50 Samples)

For Research Use Only. Not for use in diagnostic procedures.

Overview

The CoreBio RNA Prep Library Preparation Kit is a complete, end-to-end solution for generating high-quality, sequencing-ready RNA libraries from total RNA or poly(A)-selected RNA. Designed for demanding transcriptomics applications, this kit delivers consistent, reproducible results across a broad range of RNA input quantities and qualities.

Compatible with all major next-generation sequencing platforms including Illumina NovaSeq, NextSeq, and HiSeq systems, the CoreBio RNA Prep kit enables researchers to move confidently from sample to sequence with a streamlined, optimized workflow.

Key Specifications

• Catalog Number: CBR-RNA-050
• Kit Size: 50 samples
• Input RNA: 10 ng – 1 µg total RNA or 1 ng – 100 ng poly(A) RNA
• RNA Integrity: Compatible with RIN ≥ 7 (high-quality) and degraded RNA (RIN 2–6)
• Library Insert Size: 150–400 bp (default); adjustable by fragmentation time
• Sequencing Compatibility: Illumina NovaSeq 6000/X, NextSeq 550/2000, HiSeq 2500/4000
• Read Type: Single-end or paired-end
• Recommended Read Length: 2 × 75 bp or 2 × 150 bp
• Storage Conditions: –20°C; stable for 12 months from date of manufacture

Workflow Overview

1. RNA Fragmentation — Thermally fragment RNA to target insert size
2. First-Strand cDNA Synthesis — Reverse transcription using random hexamer priming
3. Second-Strand Synthesis — Generate double-stranded cDNA
4. End Repair & A-Tailing — Prepare blunt-ended fragments for adapter ligation
5. Adapter Ligation — Ligate indexed sequencing adapters
6. Library Amplification — PCR enrichment of adapter-ligated fragments
7. Library QC — Assess size distribution and quantification prior to sequencing

Applications

• Whole transcriptome sequencing (RNA-Seq)
• Gene expression profiling
• Differential expression analysis
• Alternative splicing studies
• Non-coding RNA discovery
• Pathway and network analysis

Kit Contents

• RNA Fragmentation Buffer
• First-Strand Synthesis Mix
• SuperScript Reverse Transcriptase (included)
• Second-Strand Synthesis Buffer and Enzyme Mix
• End Repair and A-Tailing Buffer and Enzyme Mix
• Ligation Buffer and DNA Ligase
• CoreBio Indexed Adapters (Set A, 50 indices)
• PCR Master Mix
• Purification Beads
• Resuspension Buffer

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